One of the primary safety devices in a laboratory is a chemical fume hood. A well-designed hood, when properly installed and maintained, can offer a substantial degree of protection to the user, provided that it is used appropriately and its limitations are understood.
This section covers a number of topics aimed at helping laboratory workers understand the limitations and proper work practices for using fume hoods and other local ventilation devices safely.
There are basically two types of fume hoods at Baylor University, they are:
Constant volume – where the exhaust flowrate or quantity of air pulled through the hood is constant. Therefore, when the sash is lowered and the cross-sectional area of the hood opening decreases, the velocity of airflow (face velocity) through the hood increases proportionally. Thus, higher face velocities can be obtained by lowering the sash.
And variable air volume (VAV) - where the exhaust flowrate or quantity of air pulled through the hood varies as the sash is adjust in order to maintain a set face velocity. Therefore, when the sash is lowered and the cross-sectional area of the hood opening decreases, the velocity of airflow (face velocity) through the hood stays the same while less total air volume is exhausted.
A fume hood is a ventilated enclosure in which gases, vapors and fumes are contained. An exhaust fan situated on the top of the laboratory building pulls air and airborne contaminants in the hood through ductwork connected to the hood and exhausts them to the atmosphere.
The typical fume hood found in Baylor University laboratories is equipped with a movable front sash and an interior baffle. Depending on its design, the sash may move vertically, horizontally or a combination of the two and provides some protection to the hood user by acting as a barrier between the worker and the experiment.
The slots and baffles direct the air being exhausted. In many hoods, they may be adjusted to allow the most even flow. It is important that the baffles are not closed or blocked since this blocks the exhaust path.
The airfoil or beveled frame around the hood face allows more even airflow into the hood by avoiding sharp curves that can create turbulence.
In most hood installations, the exhaust flowrate or quantity of air pulled through the hood is constant. Therefore, when the sash is lowered and the cross-sectional area of the hood opening decreases, the velocity of airflow (face velocity) through the hood increases proportionally. Thus, higher face velocities can be obtained by lowering the sash.
A fume hood is used to control exposure of the hood user and lab occupants to hazardous or odorous chemicals and prevent their release into the laboratory. A secondary purpose is to limit the effects of a spill by partially enclosing the work area and drawing air into the enclosure by means of an exhaust fan. This inward flow of air creates a dynamic barrier that minimizes the movement of material out of the hood and into the lab.
In a well-designed, properly functioning fume hood, only about 0.0001% to 0.001% of the material released into the air within the hood actually escapes from the hood and enters the laboratory.
When is a Fume Hood Necessary?
The determination that a fume hood is necessary for a particular experiment should be based on a hazard analysis of the planned work. Such an analysis should include:
The level of protection provided by a fume hood is affected by the manner in which the fume hood is used. No fume hood, however well designed, can provide adequate containment unless good laboratory practices are used, as follow:
Items contaminated with odorous or hazardous materials should be removed from the hood only after decontamination or if placed in a closed outer container to avoid releasing contaminants into the laboratory air.
When using cylinders containing highly toxic or extremely odorous gases, obtain only the minimal practical quantity. Consider using a flow-restricting orifice to limit the rate of release in the event of equipment failure. In some circumstances, exhaust system control devices or emission monitoring in the exhaust stack may be appropriate.
To optimize the performance of the fume hood, follow the practices listed below:
Images from Kewaunee Fume Hoods
Images from Kewaunee Fume Hoods
Images from Kewaunee Fume Hoods
Used appropriately, a fume hood can be a very effective device for containment hazardous materials, as well as providing some protection from splashes and minor explosions. Even so, the average fume hood does have several limitations.
Fume hoods are designed specifically to provide ventilation for the protection of lab occupants during chemical manipulations. The airflow they provide is greatly in excess of that needed for storage of closed containers of even the most toxic of volatile materials. Storing materials in this way is, therefore, a misuse of an expensive piece of equipment.
In general, the storage of chemicals in fume hoods is strongly discouraged. See Flammable Materials for more information about proper storage of flammable, toxic, or odorous chemicals.
The realities of available space and equipment in some laboratories may make it difficult or impossible to completely prohibit the use of hood workspaces for storage. In such a case, the following general policy is recommended:
Hoods Actively in Use for Experimentation
Storage of materials should be minimized or eliminated altogether. Materials stored in the hood can adversely affect the containment provided. In addition, the hood is frequently the focus of the most hazardous activities conducted in the laboratory. The presence of stored flammable or volatile, highly toxic materials can only exacerbate the problems resulting from an explosion or fire in the hood. Even if they are not directly involved in such an event, attempts to control or extinguish a fire may result in the spilling of stored materials.
Hoods Not in Active Use
Materials requiring ventilated storage (e.g., volatile and highly toxic, or odorous substances) may be stored in a hood if they are properly segregated and the hood is posted to prohibit its use for experimental work.
Most fume hoods at Baylor University are equipped with some type of continuous airflow monitoring device, either in the form of a magnehelic gauge or a face velocity monitor. Some are equipped with alarms.
Each hood also has a survey sticker with important information to help determine whether the particular hood is functioning properly and is appropriate for the work to be performed.
Static Pressure Gauge (Magnehelic)
Some fume hoods on campus may be equipped with static pressure gauges that measure the difference in static pressure across an orifice in the duct, or between the laboratory and the fume hood exhaust duct. Most of the devices are aneroid pressure gauges, such as magnehelics, that are mounted on the front of the hood above the sash.
The gauge is a flow rate indicator with a scale that reads in units of pressure, rather than velocity. Changes in the magnehelic reading are not linearly proportional to changes in face velocity; therefore it should only be used as an index of hood performance.
The magnehelic gauge reading at the time of the most recent hood survey is shown on each fume hood evaluation sticker. A difference of 15% or more in the magnehelic reading from that shown on the sticker is an indication that the flow rate in the duct, and thus the face velocity, may have changed significantly since the last survey. If the user notices such a change, or has any other reason to suspect that the hood is not operating properly, contact EHS for a re-survey of the hood.
Face Velocity Monitors
Many of the newer hoods have constant face velocity measuring devices. An LED readout of the face velocity is found on the device on the top corner of the hood opening. The readout indicates the actual face velocity of the hood.
Every chemical fume hood on campus should have a survey sticker affixed to the front of the hood in a conspicuous location. The sticker contains basic information about hood performance as of the most recent survey and should be consulted each time the hood is used.
The Date is the date of the last hood survey. Hoods that have not been surveyed within the past year should not be used until tested by EHS.
The Hood Number is a unique identifier for the particular hood. Refer to this number when discussing problems with a particular hood.
The Average Velocity Reading (Avg Vel) is the reading of the magnehelic gauge or other continuous monitoring device at the time of the survey.
If hood performance is judged to be unsuitable for use with hazardous chemicals, a sticker with this information is placed on the hood instead of the survey sticker.
Do not use a hood that does not have a survey sticker. If a survey is needed, call EHS.
EHS surveys each fume hood annually. The face velocity of the fume hood is measured with the sash in the Standard Operating Configuration (SOC). The inspection sticker is positioned on the hood so the arrow is in the proper location for the maximum safe sash position. The reading of the continuous monitoring device is recorded on the hood sticker.
After each performance survey, a written report of the results is furnished to the individual responsible for the hood (e.g., the Principal Investigator or laboratory manager), the Chemical Hygiene Officer for the department, and the Facilities staff for the laboratory building.
When Problems are Noted
There are several factors that can affect the performance of the hood, resulting in low face velocity or turbulent airflow. These include mechanical problems or exhaust slots blocked by large objects or excessive storage.
If a problem is found during the hood survey, a written notice will be provided on-site to the laboratory or taped to the sash of the fume hood. If the problem requires the need for work practice changes (e.g., blocked exhaust slots or excessive storage), the laboratory worker should make the recommended changes and call EHS to have the hood resurveyed.
If maintenance is necessary, the laboratory worker may initiate a work order through their department to request maintenance. EHS does not initiate maintenance or ensure that it is completed. Facilities will contact EHS when the work is complete to have the hood resurveyed.
Providing maintenance for fume hoods is a function of the Facilities Department, and is performed by Facilities personnel. Since the hood user is the person most aware of how a hood is being used on a day to day basis, it is the responsibility of the hood user to determine that maintenance is necessary and to request that it be performed.
If a hood user believes that the hood is not performing adequately, the following steps should be taken:
Many laboratories use equipment and apparatus that can generate airborne contaminants, but cannot be used within a fume hood. Examples include gas chromatographs, ovens, and vacuum pumps.
Other types of local exhaust ventilation systems may be required to control contaminants generated by these operations. Such systems must not be installed without explicit approval of the building facility manager and/or maintenance personnel. See Common Misuses of a Fume Hood for more information.
A snorkel is a flexible duct or hose connected to an exhaust system. It can only capture contaminants that are very close to the inlet of the hose, typically less than a distance equal to one half of the diameter of the duct.
Snorkels can be effective for capturing discharges from gas chromatographs, pipe nipples or the end of tubing. However, the effectiveness of the elephant trunk should be carefully evaluated before they are used to control releases of hazardous substances.
A canopy hood in a laboratory is constructed in a similar fashion to the overhead canopy hoods seen in kitchens. In order for the canopy hood to be able to capture contaminants, the hood requires a relatively large volume of air movement, making them somewhat costly to operate. The canopy hood works best when the thermal or buoyant forces exist to move the contaminant up to the hood capture zone.
One of the biggest problems with canopy hoods is that, in most cases, they are designed such that the contaminated air passes through the individual's breathing zone. The airflow is easily disrupted by cross currents of air.
For the most part, canopy hoods should only be used for exhaust of non-hazardous substances.
Highly toxic or odorous gases should be used and stored in gas cabinets. In the event of a leak or rupture, a gas cabinet will prevent the gas from contaminating the laboratory.
Gas cabinets should be connected to laboratory exhaust ventilation using hard duct, rather than snorkels, since such tubing is more likely to develop leaks.
There are two general types of glove boxes, one operating under negative pressure, the other operating under positive pressure. Glove boxes consist of a small chamber with sealed openings fitted with arm-length gloves. The materials are placed inside the chamber and manipulated using the gloves.
A glove box operating under negative pressure is used for highly toxic gases, when a fume hood might not offer adequate protection. A rule of thumb is that a fume hood will offer protection for up to 10,000 times the immediately hazardous concentration of a chemical. The airflow through the box is relatively low, and the exhaust usually must be filtered or scrubbed before release into the exhaust system.
A glove box operating under positive pressure may be used for experiments that require protection from moisture or oxygen. If this type of glove box is to be used with hazardous chemicals, the glove box must be tested for leaks before each use. A pressure gauge should be installed to be able to check the integrity of the system.
A conventional fume hood should not be used for work with viable biological agents. A biosafety cabinet is specially designed and constructed to offer protection to both the worker and the biological materials.
Similarly, a biosafety cabinet should generally not be used for work with hazardous chemicals. Most biosafety cabinets exhaust the contaminated air through high efficiency particulate air (HEPA) filters back into the laboratory.
This type of filter will not contain most hazardous materials, particularly gases, fumes or vapors. Even when connected to the building exhaust system, a ducted biosafety cabinet may not achieve a face velocity of 95 - 125 feet per minute, making it inappropriate for use with hazardous chemicals.
Use of a "ductless fume hood" is strongly discouraged. These devices work by using a fan to draw air into a chamber, through one or more filters, and back into the laboratory. EHS and several professional safety and engineering organizations do not recommend the use of ductless fume hoods for several reasons. First, it is difficult to determine whether the filters are functioning adequately or need to be changed; thus, the potential for recirculating toxic materials into the laboratory is significant. In the event of a chemical spill, the hood is usually not able to contain the spilled material or the potentially high concentrations of chemical vapors.
Second, the face velocity of the hood is normally below 80 feet per minute. The hood is normally designed such that the air does not flow smoothly and evenly through the hood. Both of these characteristics make it likely for disruption of airflow or turbulence, causing unfiltered air to leak into the laboratory.
Clean benches are similar to appearance as a fume hood; however, they do not exhaust air from the laboratory. A clean bench is a device that draws air from the lab through a HEPA filter and vents the filtered air downwards onto a work surface to keep the materials within free from particulate contamination. These devices are not to be used with hazardous materials as they provide no personal protection. Do not store materials on top of this hood as this will block the filter, overload the motor, and provide poor product protection.
The Standard Operating Configuration (SOC) is the position at which the hood sash should be placed when the hood is actually in use as a containment device. Making such an assumption is unavoidable when designing a fume hood exhaust system since this determines the quantity of air the system must exhaust if an adequate face velocity is to be maintained.
In order to obtain the recommended face velocity, many fume hoods have an SOC which is less than a fully open sash. If a hood user does not use the hood with the sash at the SOC position, it is possible for that user to create a situation in which an otherwise properly operating hood has an insufficient face velocity. Some fume hoods are equipped with sash stops and/or alarm devices to designate the Standard Operating Configuration limit.
Each chemical fume hood at the University has an assigned Standard Operating Configuration. Listed below are brief descriptions and the SOC’s of several styles of fume hoods commonly found at the University. Questions about the SOC of a specific hood may be addressed to the Office of Environmental Health and Safety.
Vertical Sash - A single vertical rising sash with a maximum face opening about 30 inches high. This style is sometimes modified as a distillation hood, in which case the maximum face opening is greater. The SOC is a fully open sash, unless the hood is alarm equipped. Then the SOC is the point just before the alarm is engaged.
SOC for Vertical and Double Vertical Sash (numbers are measuring points)
Double-Vertical Sash - Two vertical rising sashes side-by-side. The SOC is generally both sashes fully open. A few older installations may have an SOC of one sash closed and the other fully open.
Horizontal Sash - Two or more horizontal sliding sashes. The height of the face opening is approximately 30 inches and the maximum opening width is 1/3 to ½ of the width of the hood. This style hood is rarely equipped with an air by-pass. It is sometimes modified as a chromatography hood, in which case the height of the face opening is greater. The SOC is the largest opening that can be obtained without removing any sashes from their tracks.
SOC for Horizontal Sash (numbers are measurement points)
Combination Sash - A vertical rising sash in which two or more horizontal sliding panels are mounted. The SOC is a fully raised sash with horizontal panels fully closed, unless the hood is alarm equipped. Then the SOC is fully closed horizontal panels with the vertical sash raised to just below the point at which the alarm is engaged. A few old installations have an SOC of vertical sash down, horizontal panels open as much as possible without removing them.
Walk-In Hood - a maximum face opening six feet or more high and extends to floor level. At Baylor, this style is usually equipped with two vertical rising sashes mounted in parallel tracks and each capable of closing half the face opening. The SOC is one half of the face open.
California Hood - a free standing bench top enclosed on all sides by transparent material for a height of four feet or more above the bench, and ventilated. Horizontal sliding doors provide access from two opposite sides. The SOC is all doors closed.
Triple-Vertical Sash - three adjacent vertical-rising sashes, the center one of which is 18" wide, in an otherwise standard vertical sash hood. The SOC is center sash down and sashes on both sides fully raised.
SOC for Triple Vertical Sash (numbers are measurements points)