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Cell Culture Facilities


The MBC currently has three cell culture facilities available for use. These facilities are located in C305R.3, D309R.1, and D309R.2.


C305R.3 BSL-1 Cell Culture Facility

The C305R.3 cell culture facility is located within the main lab facility of the MBC. To obtain access to this facility, you will need approved card access to the MBC.

Room C305R.3 is outfitted with two certified Class II Type A/B3 Laminar flow hoods, one CO2 incubator, one tabletop centrifuge, one inverted scope, one designated set of micropipettors, one designated Pipet-Aid, and a sink for handwashing. Because this facility is incorporated into the main MBC lab, this is an ideal area for undergraduate and individuals inexperienced in cell culture to work, as the Director of the facility is located close by to address questions/concerns. The MBC can offer basic training on good cell culture practices; however, in-depth cell culture training for graduate students/undergraduates/other researchers/etc, is the responsibility of the individual research lab. Standard operating procedures are in place for this facility and will need to be reviewed by any individuals interested in using the facility prior to conducting research.

As a BSL-1 cell culture facility, absolutely no organisms classified at the BSL-2 level may be handled directly or indirectly in this cell culture facility. This includes work involving human cell lines. Research involving human cell lines is prohibited in the C305R.1 cell culture facility as this research is classified, at a minimum, at the BSL-2 level by Baylor University's Institutional Biosafety Committee.


D309R.1 BSL-2 Cell Culture Facility

The D309R.1 cell culture facility is accessed through the Sterilizer Facility (D307). To obtain access to the cell culture facility, you will need approved card access to the Sterilizer Facility. In addition to approved card access to the Sterilizer Facility, D309R.1 is a keyed room and you will need key access in order to use the facility (please contact the MBC Director at Michelle_Nemec@baylor.edu).

For new research to take place in this facility, the appropriate documentation and manuals will have to be approved by Baylor University's Institutional Biosafety Committee and submitted to the Director of the MBC prior to beginning research. For more details on this process and if this facility can accommodate your research, please contact the MBC Director.


D309R.2 BSL-1 or BSL-2 Cell Culture Facility

The D309R.2 cell culture facility is accessed through the Sterilizer Facility (D307). To obtain access to the cell culture facility, you will need approved card access to the Sterilizer Facility. In addition to approved card access to the Sterilizer Facility, D309R.2 is a keyed room and you will need key access in order to use the facility (please contact the MBC Director at Michelle_Nemec@baylor.edu).

Room D309R.2 is outfitted with two certified Class II Type A/B3 Laminar flow hoods, one CO2 incubator, one tabletop centrifuge, one inverted scope, one designated set of micropipettors, one designated Pipet-Aid, and a sink for handwashing. Because this facility is not located within the main MBC lab and the MBC Director is not close by for assistance, this area is ideal for researchers/students who already have experience in cell culture (Room C305R.3 is a more appropriate work area for those who are just learning cell culture techniques). Standard operating procedures are in place for this facility and will need to be reviewed by any individuals interested in using the facility prior to conducting research.

At present, this facility operates at the BSL-1 level. Absolutely no agents classified at the BSL-2 level may be handled directly or indirectly in this cell culture facility at this time. This includes work involving human cell lines. The D309R.2 Cell Culture Facility can be reclassified as a BSL-2 cell culture facility if the need is present. The research taking place, as well as the appropriate documentation and manuals, will have to be approved by Baylor University's Institutional Biosafety Committee and submitted to the Director of the MBC prior to beginning research. For more details on this process, please contact the MBC Director.