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Cell Culture Facilities

Cell Culture Facility

The MBC currently has three shared cell culture facilities available for use. These shared facilities are located in C305R.3, D309R.1, and D309R.2.


C305R.3 BSL-1 Cell Culture Facility

The C305R.3 shared cell culture facility is located within the main lab facility of the MBC and is limited to Biosafety level 1 research (as classified by Baylor’s Institutional Biosafety Committee) at this time. To obtain access to this facility, you will need approved card access to the MBC.

Room C305R.3 is outfitted with two certified Class II Type A/B3 Laminar flow hoods, one CO2 incubator, one tabletop centrifuge, one inverted microscope, one designated set of micropipettors, one designated Pipet-Aid, and a sink for handwashing. Because this facility is incorporated into the main MBC lab, this is an ideal area for undergraduate and individuals inexperienced in cell culture to work, as the Director of the facility is located close by to address questions/concerns. The MBC can offer basic training on good cell culture practices; however, in-depth cell culture training for graduate students/undergraduates/other researchers/etc, is the responsibility of the individual research lab. Standard operating procedures are in place for this facility and will need to be reviewed by any individuals interested in using the facility prior to conducting research.

As a BSL-1 cell culture facility, absolutely no organisms classified at the BSL-2 level may be handled directly or indirectly in this cell culture facility.


D309R.1 BSL-2 Cell Culture Facility

The D309R.1 shared cell culture facility is accessed through the Sterilizer Facility (D307), is classified as a Biosafety Level 2 work area and is currently at capacity. To obtain access to this cell culture facility, you will need approved card access to the Sterilizer Facility. Additionally, D309R.1 is a keyed room and you will need key access in order to use the facility (please contact the MBC Director at Michelle_Nemec@baylor.edu). Bloodborne Pathogen Training is also required prior to entry into this facility. For more details on this shared cell culture space, please contact the MBC Director.


D309R.2 BSL-1 or BSL-2 Cell Culture Facility

The D309R.2 shared cell culture facility is accessed through the Sterilizer Facility (D307). It is outfitted with two certified Class II Type A/B3 Laminar flow hoods, one CO2 incubator, one tabletop centrifuge, one inverted microscope, one designated set of micropipettors, one refrigerator/freezer, one waterbath and a sink for handwashing To obtain access to the cell culture facility, you will need approved card access to the Sterilizer Facility. Additionally, D309R.1 is a keyed room that remains locked at all time. You will need key access in order to use the facility (please contact the MBC Director at Michelle_Nemec@baylor.edu.

Biosafety level 2 research involving human cell lines is currently being conducted in this shared space. For new research projects to take place in this facility, the appropriate documentation and manuals will have to be approved by Baylor University's Institutional Biosafety Committee and submitted to the Director of the MBC prior to beginning research. Bloodborne Pathogen Training is also required prior to entry into this facility. For more details on this process and if this facility can accommodate your research, please contact the MBC Director.