Baylor > BSB > Mass Spectrometry Center > Instrumentation

Instrumentation


Currently there are ten mass spectrometers accessible to the Baylor Science Building supported by the Center:

Synapt G2-S• Waters Synapt G2-S HDMS (Rooms E155R and C153): High resolution mass spectra (up to 40,000) can be obtained using two identical Waters Synapt G2-S mass spectrometers. Ions can be separated in this mass spectromer by quadrupole time-of-flight mass spectrometry (QTOF) or/and by gas-phase ion mobility separation with resolving power > 40 (Ω/ΔΩ full width half maximum). Samples can be introduced to the G2-Ss by direct infusion or by attached nano Acquity-UPLC systems.











Synapt• Waters Synapt G1 HDMS(Room E156R): High resolution mass spectra (up to 17,000) can be obtained using a Waters Synapt G1 HDMS. Samples can be introduced to the Synapt by direct infusion or by an attached Acquity-UPLC system.








ProSpec• Waters Prospec (Room E156R): High Resolution mass spectra (up to 10,000) can be obtained using a VG Prospec Micromass spectrometer using electron ionization (EI) or chemical ionization (CI). Samples can be introduced by direct insertion or gas chromatography.








Xevo TQS• Waters Xevo TQ-S (Room E156R): Low resolution mass spec data can be obtained in a triple quadrupole Xevo TQ-S mass spectrometer. Samples can be introduced to the TQ-S by direct infusion or by an attached Acquity-UPLC system.







Orbitrap• Thermo Orbitrap Discovery (Room E156R): High resolution mass spectra (up to 30,000) can be obtained using a Thermo scientific LTQ Orbitrap Discovery. Samples can be introduced by direct infusion or by the use of an Accela Bundle LC system. Modes of ionization are electrospray ESI, APCI/APPI and nano-ESI. Important upgrades include the acquisition of Proteome Discoverer and Sieve for proteomic analysis.






DSQ• Thermo DSQ (Room E207): Unit resolution EI mass spectra can be obtained using a Thermo Scientific DSQ II. Samples can be introduced by direct insertion or gas chromatography. The DSQ is used for the advance Organic lab, but can be accessible to the BSB after training.








Varian 1200• Varian LC-MS/MS 1200L (Room E159R): The Center supports one low resolution Varian QqQ 1200L. Samples can be introduced to the 1200L Varian by direct infusion or by the use of a Varian LC system.









Saturn• Varian IT-MS Saturn 2100T (Room A455): The Center supports a low resolution Varian IT Saturn 2100T. Samples can be introduced Saturn by a Varian 3900 chromatograph.









ICP-MS• Perkin Elemer ICP-MS ELAN 9000 (Room E159R): The Center supports a low resolution Perkin Elmer ICP-MS. This instrument can be used for trace level analysis of metals (part-per-trillions level).