Currently there are eight mass spectrometers accessible to the Baylor Science Building supported by the Center:
• Waters Synapt G2-S HDMS (Rooms E155R and C153): High resolution mass spectra (over 40,000) can be obtained using two identical Waters Synapt G2-S HDMS mass spectrometers. Ions can be separated in these two mass spectrometers by quadrupole time of flight mass spectrometry or/and by gas-phase ion mobility separation with resolving power > 40 (Ω/ΔΩ full width half maximum). Samples can be introduced into the two G2-S mass spectrometers by direct infusion or by the attached nano Acquity-UPLC systems. Data generated in any of the Waters mass spectrometers present in the center can be analyzed in a dedicated processing computer that includes Protein Global Lynx Server (PLGS) intended for bottom up proteomic analysis.
• Waters Synapt HDMS (Room E156R): High resolution mass spectra (up to 17,000) can be obtained using a Waters Synapt HDMS. Ions can be separated in the Synapt mass spectrometer by quadrupole time of flight mass spectrometry or/and by gas-phase ion mobility separation. Samples can only be introduced into the Synapt by direct infusion.
• Thermo Orbitrap Discovery (Room E156R): High resolution mass spectra (up to 30,000) can be obtained using a Thermo scientific LTQ Orbitrap Discovery. Samples can be introduced by direct infusion. Modes of ionization include electrospray ESI, APCI/APPI and nano-ESI. Data generated in the Orbitrap mass spectrometer can be analyzed in a dedicated processing computer that includes Proteome Discoverer (proteomic bottom up analysis) and Sieve (identification of samples with statistical inter-sample differences).