Instrumentation

Currently there are six mass spectrometers accessible to the Baylor Science Building and three mass spectrometers of individual use (also accessible) supported by the Center:

• Waters Prospec (open access): High Resolution mass spectra (up to 10,000) can be obtained using a VG Prospec Micromass spectrometer using electron ionization (EI) or chemical ionization (CI). Samples can be introduced by direct insertion or gas chromatography. This instrument is accessible to faculty and student after training.

• Thermo Orbitrap (open access): High resolution mass spectra (up to 30,000) can be obtained using a Thermo scientific LTQ Orbitrap Discovery. Samples can be introduced by direct infusion or by the use of an Accela Bundle LC system. Modes of ionization are electrospray ESI, APCI/APPI and nano-ESI. Important upgrades include the acquisition of Proteome Discoverer and Sieve for proteomic analysis. This instrument is accessible to faculty and student after training.

• Waters Synapt (open access): High resolution mass spectra (up to 17,000) can be obtained using a Waters Synapt HDMS. Samples can be introduced to the Synapt by direct infusion or by an attached Acquity-UPLC system. An important feature on this system beside the presence of a Q-TOF in W mode is the addition of an ion mobility cell that allows separation of molecules in another dimension of sample separation. This instrument is accessible to faculty and student after training.

• Thermo DSQ (open access): Unit resolution EI mass spectra can be obtained using a Thermo Scientific DSQ II. Samples can be introduced by direct insertion or gas chromatography. The DSQ is used for the advance Organic lab, but can be accessible to the BSB after training.

• Thermo LCQ (open access): Unit resolution ESI mass spectra can be obtained using a Thermo LCQ Classic. Samples can be introduced by direct infusion or liquid chromatography. Open access is provided after training.

• Varian LC-MS/MS 1200L: The Core facility supports two low resolution Varian QqQ 1200L. Samples can be introduced to the 1200L Varians by direct infusion or by the use of two Varian LC systems.

• Varian IT-MS Saturn 2100T: The Core facility supports a low resolution Varian IT Saturn 2100T. Samples can be introduced Saturn by a Varian 3900 chromatograph.

• Perkin Elemer ICP-MS ELAN 9000: The Core facility supports a low resolution Perkin Elmer ICP-MS. This instrument can be used for trace level analysis of metals (part-per-trillions level).